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Antibodies sure to the blocking peptide not bind into the epitope about the target protein. By evaluating the staining in the blocked antibody vs . the antibody on your own, you can see which staining is distinct.
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This information outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
This tutorial outlines further attainable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind especially for the goal get more info antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S phase boundary and is degraded as cells progress through S period.
Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial purpose in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been explained. Two additional splice variants had been described but detailed nucleotide sequence info isn't nevertheless available.
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